provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. Using the Retro-X Concentrator protocol, we were able to increase the titer of retroviral supernatants for various envelope-pseudotyped retrovirus samples from 46-fold (amphotropic and 10A1 pseudotypes) to 100-fold (ecotropic and VSV-G), when concentrating them from 10 ml down to 100 µl. At Takara Bio, scalability has been successful for supernatant volumes up to 250 ml. The Retro-X Concentrator reagent is a 4X concentrate, so it can be added to any volume of supernatant containing any amount of virus or any starting titer. Scalable for supernatants of any volume, any titer Far simpler than ultracentrifugationĬompared to ultracentrifugation, the Retro-X Concentrator is more flexible in terms of scale, is easier to use than ultracentrifugation, and requires no specialized equipment. You’ll increase your retroviral titer by up to 100-fold and obtain excellent recoveries-with no ultracentrifugation. In the simple protocol, you just mix your retroviral supernatant with the retrovirus concentration reagent, incubate for a short period, and spin the mixture in a standard centrifuge. Retro-X Concentrator provides a fast, simple, and highly efficient method for concentrating any retroviral stock including MMLV- or MSCV-based retroviral supernatants.
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